Control:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Over Expression:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Generated:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Transduction:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Plasmid Preparation:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Construct:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Single Cell:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
Clone Assay:Article Title: Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus.
Article Snippet: .. A549-IRF3 KO cells were described in Aarreberg et al.63; A549-RML cells were described in Esser-Nobis et al.33; A549 non-targeting control (NTC), A549-MDA-5 KO, and A549RIG-I KO cells were described in Esser-Nobis et al.26 A549-RIG-I OE (RIG-I overexpression cells) were generated by lentiviral transduction of cells with a selectable pWPI lentiviral vector construct encoding RIG-I (pWPI_RIG-I_blr, a kind gift from Ralf Bartenschlager), selected for transduction with blasticidin 10mg/mL (Invivogen), then single-cell cloned by limiting dilution. .. All cell lines were routinely confirmed negative for mycoplasma contamination by MycoAlert test kit (Lonza) and maintained at 37 C and 5% CO2.
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